DNA Vectors
System Biosciences LLC pCDH-EF1α-MCS-(PGK-GFP) Bidirectional Promoter Cloning and Expression LentivectorpCDH-EF1α-MCS-(PGK-GFP) Bidirectional Promoter, Cloning and Expression Lentivector = 10ug
Get another option for robust, coordinated dual gene expression with the pCDH-EF1α-MCS-(PGK-GFP) Bidirectional Promoter Lentivector. Well-regarded in the industry for reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-EF1α-MCS-(PGK-GFP) Bidirectional Promoter Cloning and Expression Lentivector gives you another option for robust, coordinated expression of your gene-of-interest and a GFP reporter.
System Biosciences LLC pCDH-MSCV-MCS-EF1α-GFP Cloning and Expression LentivectorpCDH-MSCV-MCS-EF1α-GFP cDNA Dual Promoter Cloning and Expression Lentivector = 10ug
The pCDH-MSCV-MCS-EF1α-GFP lentivector uses the MSCV promoter for high expression in hematopoietic or stem cells and includes a GFP reporter. Well-regarded in the industry for reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-MSCV-MCS-EF1α-GFP Cloning and Expression Lentivector drives expression of your gene-of-interest from the MSCV promoter, which provides strong expression in hematopoietic and stem cells. This lentivector also enables selection for transductants using a GFP reporter.
System Biosciences LLC pCDH-CMV-MCS-EF1α-RFP Cloning and Expression LentivectorpCDH-CMV-MCS-EF1α-RFP cDNA Dual Promoter Cloning and Expression Lentivector = >1E6 IFUs
With the strong CMV promoter upstream of an MCS and EF1α driving the RFP reporter, pCDH-CMV-MCS-EF1α-RFP is a great, all-purpose lentivector. Well-regarded in the industry for high, reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-CMV-MCS-EF1α-RFP Cloning and Expression Lentivector drives expression of your gene-of-interest from the strong CMV promoter, and expression of the RFP reporter from the EF1α promoter.
System Biosciences LLC PGREENFIRE1-LXRE IN LXRA HEPG2 CELL LINE
Study cholesterol homeostasis by co-expressing dscGFP and luciferase in response to Liver X Receptor (LXR) activity at the LXRα promoter in HepG2 Cells. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid*, ready-to-transduce pre-packaged lentivirus, and as a ready-to-study cell line. The pGreenFire1-LXRE-in-LXRα Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from the Liver X Receptor (LXR) response elements (LXREs) and neighboring regions in the LXRα promoter paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of the LXRE/LXRα transcriptional elements, drives expression of dscGFP and luciferase in response to LXR activity. The result is the ability to quantitatively measure LXR activity at the LXRα promoter by fluorescence and luciferase activity.
System Biosciences LLC MSCV-hspCas9-EF1α-GFP Two Vector Cas9 SmartNuclease™ LentivectorCas9 expression vector for the Two Vector SmartNuclease System (MSCV-hspCas9-EF1α-GFP Lentivector Pre-Packaged Lentiviral Particles = >10^6 IFUs
When you want to create stable Cas9 editing cell lines and/or would like to edit the genome of a cell line that is resistant to transfection by plasmids, turn to one of SBI’s Cas9 SmartNuclease Lentivector Systems. Available as lentivector plasmids, ready-to-transduce pre-packaged pseudovirus, and in a package-your-own-lentivector kit with the lentivector plasmid and the LentiStarter 3.0 Packaging Kit, the MSCV-hspCas9-EF1α-GFP Two Vector Cas9 SmartNuclease™ Lentivector expresses human codon-optimized Cas9 wild-type nuclease from the strong MSCV promoter. The vector also includes a GFP marker expressed from the EF1α promoter to verify transduction efficiencies.